Phytotoxicity and biodirected fractionation of extracts of barks of Blepharocalyx salicifolius (Kunth) O.Berg. (Myrtaceae)

Authors

  • Eduardo Habermann Universidade Federal de São Carlos - UFSCar
  • Viviane de Cassia Pereira UFSCar
  • Maristela Imatomi UFSCar
  • Flávia Cevithereza Pontes UFSCar
  • Sonia Cristina Juliano Gualtieri Professora titular

DOI:

https://doi.org/10.5007/2175-7925.2015v28n1p37

Abstract

This study aimed to evaluate the phytotoxicity of extracts and fractions of barks of Blepharocalyx salicifolius on elongation of etiolated coleoptiles of Triticum aestivum (wheat). The extracts hexane (Hx), ethyl acetate (AcOET), and aqueous (H2O) were obtained by means of exhaustive extraction with CH2Cl2/CH3OH and subsequent fractionation by partition chromatography coefficient. The extracts AcOET and Hx were fractionated by column chromatography by using organic solvents in increasing order of polarity yielding 7 hexanic fractions and 6 ethylic acetate fractions. Subsequently, the fractions Hx1 and Hx5 were subfractionated by column chromatography. The extracts AcOET and Hx inhibited elongation of coleoptiles. Four ethylic acetate fractions inhibited elongation of coleoptiles at all concentrations. Five hexanic fractions inhibited elongation of coleoptiles, the fractions Hx5 and Hx1 fractions showed phytotoxic effects equivalent or superior to those observed by the herbicide GOAL® at the same concentrations. All subfractions obtained by means of fractionation of Hx1 inhibited elongation of coleoptiles. Six fractions obtained by means of fractionation of Hx5 inhibited elongation of coleoptiles at all concentrations. This study proves the phytotoxicity of extracts of barks of B. salicifolius, providing indications that they may act as promising natural herbicides.

Author Biographies

Eduardo Habermann, Universidade Federal de São Carlos - UFSCar

Graduando em Ciências Biológicas pela Universidade Federal de São Carlos, campus São Carlos

Viviane de Cassia Pereira, UFSCar

Ecóloga, Mestre em Ecologia e Recursos Naturais.

Maristela Imatomi, UFSCar

Bióloga, Mestre e doutora em Ecologia e Recursos Naturais.

Flávia Cevithereza Pontes, UFSCar

Bióloga, Mestranda em Ecologia e Recursos Naturais

Sonia Cristina Juliano Gualtieri, Professora titular

Possui graduação em Licenciatura e Bacharelado em Ciências Biológicas pela Universidade Federal de São Carlos (1979), graduação em Licenciatura e Bacharelado em Ciências Biológicas pela Universidade Federal de São Carlos (1979), mestrado em Ecologia e Recursos Naturais pela Universidade Federal de São Carlos (1984) e doutorado em Ecologia e Recursos Naturais pela Universidade Federal de São Carlos (1988). 

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Published

2014-10-18

Issue

Vol. 28 No. 1 (2015)

Section

Artigos

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