Variability and molecular genetic structure in Passiflora edulis Sims. accessions based on markers analogous to resistance genes
DOI:
https://doi.org/10.5007/2175-7925.2023.e91095Keywords:
Diversity, Genetic resources, Germplasm, Molecular marker, Passion fruit vineAbstract
Brazil is a center of diversity of passion fruit vine species, with Passiflora edulis Sims. being the most cultivated one. Due to their commercial importance and susceptibility to diseases, the objective of this study was to evaluate the genetic diversity and structuring of Passiflora edulis Sims. accessions from the Cerrados and Mandioca & Fruticultura active germplasm banks of Embrapa using Resistance Gene Analog (RGA) molecular markers. The genetic diversity of 163 plants, totaling 71 unimproved varieties and five cultivars, was evaluated. Nine combinations of primers were used, totaling 157 markers, with an average of 17.4 markers/combination. The mean expected heterozygosity was 0.18. The polymorphic information content was 0.15. The molecular variance showed 82% diversity existing within the Active Germplasm Banks (AGBs). The genotypes were structured in three gene pools, with a heterogeneous subgroup containing accessions from both banks, a homogeneous one with accessions from he Cerrados ABG and the last one containing mainly cultivars from the same. Principal Coordinate Analysis (PCoA) demonstrated the banks have similar genotypes. The results obtained emphasized the existence of passive genomic material for the development of new cultivars. The markers certify that these collections can contribute to breeding programs. There are shared alleles between unimproved varieties of the two ABGs; however, these varieties are not related to the cultivars.
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