Genetic variability of captive populations of Rhamdia quelen (Teleostei: Pimelodidae) using microsatellite markers

Authors

  • Marina Virmond Universidade Federal do Paraná
  • Danielle Conceição Universidade Federal do Paraná
  • Hilton Amaral Junior Epagri, Empresa de Pesquisa Agropecuária de Santa Catarina, Município de Camboriú, Santa Catarina
  • Rodolfo Luis Petersen Universidade Federal do Paraná http://orcid.org/0000-0003-1048-3117

DOI:

https://doi.org/10.5007/2175-7925.2017v30n4p51

Abstract

The analysis of genetic variability in populations that are candidates for breeding programs is important for selecting a base population, which maximizes genetic divergence. Two captive populations of Rhamdia quelen were analyzed with microsatellite markers. The loci were amplified with fluorophore labelled primers: Pcor1 (FAM), Pcor2 (TET), Pc17 (HEX), Pc97 (TET), and Rh1 (FAM). The five examined microsatellite loci were highly polymorphic and informative with an average of 9.8 alleles/locus. The inbreeding coefficient (FIS) presented negative values indicating that inbreeding did not occur. The allelic and genotypic differentiation tests between the two populations showed statistically significant values; the total genetic divergence (FST) value was 0.01939, indicating a low level of genetic divergence. The analysis of molecular variance (AMOVA) showed that the highest genetic variability was within (98.06) and not between the populations. The microsatellite markers used were efficient for analyzing the structure and genetic variability in captive populations of Rhamdia quelen. The adopted reproduction management proved to be appropriate because it ensured the maintenance of genetic variability in these populations.

Author Biographies

Marina Virmond, Universidade Federal do Paraná

Oceanógrafa. Centro de Estudos do Mar, Universidade Federal do Paraná

Danielle Conceição, Universidade Federal do Paraná

Centro de Estudos do Mar, Universidade Federal do Paraná

Hilton Amaral Junior, Epagri, Empresa de Pesquisa Agropecuária de Santa Catarina, Município de Camboriú, Santa Catarina

Diretor Area de Aquicultura Epagri, Empresa de Pesquisa Agropecuária de Santa Catarina, Município de Camboriú, Santa Catarina

Rodolfo Luis Petersen, Universidade Federal do Paraná

Professor do Centro de Estudos do Mar. Setor de Ciências da Terra, UFPR. Coordenador do GECEMar, Laboratório de Melhoramento Genético de Organismos Aquáticos, Centro de Estudos do Mar, UFPR.

References

ANDRADE, D. R.; YASUI, G. S. O manejo da reprodução natural e artificial e sua importância na produção de peixes no brasil. Revista Brasileira de Reprodução Animal, Belo Horizonte, v. 27, p. 166-172, 2003.

BALDISSEROTTO, B.; NETO, J. R. Criação de jundiá. Santa Maria: Editora UFSM, 2004. 232 p.

BOTSTEIN, D.; WHITE, R. P.; SKOLNICK, M.; DAVIS, R. W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics, Houston, v. 32, p. 314-331, 1980.

CARNEIRO, P. C.; BENDHACK, F.; MILKOS, J. D.; SCHORER, M.; OLIVERA FILHO, P. R C. Jundiá: um grande peixe para a Região Sul. Panorama da Aquicultura, Rio de Janeiro, v. 12, p. 41-46, 2002.

DANTAS, H. L.; dos SANTOS, N.; ARCANJO, M.; OLIVEIRA, K.; CAVALCANTI, K.; SEVERI, W. I.; DINIZ, F.; COIMBRA, M. R M. Genetic diversity of captive and wild threatened catfish Pseudoplatystoma corruscans in the São Francisco River. Reviews in Fisheries Science, Philadelphia, v. 21, p. 237-246, 2013.

DE LEON, F. J. G.; CANONN, M.; QUILLET, E.; BONHOMME, F.; CHATAIN, K. The application of microsatellite markers to breeding programmes in the sea bass, Dicentrarchus labrax. Aquaculture, Amsterdam, v. 159, p. 303-316, 1998.

EXCOFFIER, L.; LAVAL, G.; SCHENEIDER, S. Arlequin v. 3.1.1: an integrated software package for population genetics data analysis. Evolutionary Bioinformatics, Cambridge, v. 1, p. 47-50, 2005.

FALCONER, D. S.; MACKAY, T. F. C. Introduction to quantitative genetics. 4 ed. Edinburgh: Longman Group Limited, 1996. 464 p.

FERGUNSON, A.; TAGGART, J. B.; PRODOLH, P. A.; MCMEEL, O.; THOMPSON, C.; STONE, C.; McCGINNITY, P.; HYNES, R. A. The application of molecular markers to the study and conservation of fish populations, with special reference to Salmo. Journal of Fish Biology, Malden, v. 47, p. 103-126, 1995.

FERREIRA, M. E.; GATTAPAGLIA, D. Introdução ao uso de marcadores moleculares em análise genética. 3 ed. Brasília: Embrapa-Cenargen, 1998. 220 p.

HILSDORF, A.; KRIEGER, J. E. Biologia molecular na conservação de peixes: ferramentas moleculares e conservação genética. Biotecnologia Ciência e Desenvolvimento, Natal, v. 1, p. 10-12, 1998.

KOCHER, T. D.; LEE, W. J.; SOBOLEWSKA, H.; PENMAN, D.; MCANDREW, B. A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus). Genetics, Pittsburgh, v. 148, p. 1225-1232, 1998.

LIDANI, K. C. F.; LIMA, J. R.; TORRES, R. A.; GABRIEL, J. E.; MADEIRA, H. M. F.; CARNEIRO, P. C. F. Variabilidade genética de um estoque cativo de jundiá (Rhamdia quelen). Revista Acadêmica, Curitiba, v. 4, n. 3, p. 47-53, 2006.

LITT M.; LUTY J. A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics, Houston, v. 44, p. 39-40, 1989.

LINDARI, C. F.; LIMA, J. R; TORRES, R.A.; GABRIEL, J. A.; MADEIRA, H. M. F. Variabilidade genética de um estoque cativo de jundiá (Rhamdia quelen) Rev. Acad., Curitiba, v.4, n.3, p. 47-53, 2006.

LOPERA-BARRERO, N. M.; RIBEIRO, R. P.; POVH, J. A.; SIROL, R. N.; MANGOLIN, C. A. Genetic Evaluation of pacu (Piaractus mesopotamicus) natural populations and from the broodstocks of a stock enhancement program using microsatellite markers. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 62, p. 954-963, 2010.

NETO, M. A. Avaliação genética do estoque fundador de surubim, Pseudoplatystoma corruscans (Spix & Agassiz, 1829), para o repovoamento do submédio rio São Francisco. Dissertação (Mestrado em Recursos Pesqueiros e Aquicultura) - Universidade Federal Rural de Pernambuco, Recife. 2008.

O’CONNELL, P.; WRIGHT, J. M. Microsatellite DNA in fishes. Reviews in Fish Biology and Fisheries, Washington, v. 7, p. 331-363, 1997.

O’REILLY, P.; WRIGHT, J. M. The evolving technology of DNA fingerprinting and its application to fisheries and aquaculture. Journal of Fish Biology, Malden, v. 47, p. 29-55, 1995.

OSTRENSKY, A.; BORGHETTI, J. R.; SOTO, D. Estudo setorial para consolidação de uma aquicultura sustentável no Brasil. Curitiba: Grupo Integrado de Aquicultura e Estudos Ambientais, 2007. 279 p.

PEREIRA, L. H. G.; FORESTI, F.; OLIVEIRA, C. Genetic structure of the migratory catfish Pseudoplatystoma corruscans (Siluriformes: Pimelodidae) suggests homing behavior. Ecology of Freshwater Fish, Malden, v. 18, p. 215-225, 2008.

RAYMOND, M.; ROUSSET, F. Genepop (version 1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity, Oxford, v. 86, p. 248-249, 1995.

REVALDADES, E.; PEREIRA, L. H. G.; FORESTI, F.; OLIVEIRA, C. Isolation and characterization of microsatellite loci in Pseudoplatystoma corruscans (Siluriformes: Pimelodidae) and cross-species amplification. Molecular Ecology Notes, Weinheim, v. 5, p. 463-465, 2005.

RIBOLLI, J.; MELO, M. R.; ZANIBONI-FILHO, E. Genetic characterization of the Neotropical catfish (Pimelodidae, Siluriformes) in the Upper Uruguay River. Genetics and Molecular Biology, Ribeirão Preto, v. 35, p. 761-769, 2012.

RIBOLLI, J.; ZANIBONI-FILHO, E. Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen. Neotropical Ichthyology, Maringá, v. 7, p. 629-634, 2009.

ROMANA-EGUIA, M. R. R.; IKEDA, M.; BASIAO, Z. U.; TANIGUCHI, N. Genetic diversity in farmed Asian Nile and red hybrid tilapia stocks evaluated from microsatellite and mitochondrial DNA. Aquaculture, Amsterdam, v. 236, p. 131-150, 2004.

SEKINO, M.; SUGAYA, T.; HARA, M.; TANIGUCHI, N. Relatedness inferred from microsatellite genotypes as a tool for broodstock management of Japanese flounder Paralichthys olivaceus. Aquaculture, Amsterdam, v. 233, p. 163-172, 2004.

WEBER, Z.; MAY, P. E. Abundant class of human DNA polymorphism which can be typed using the polymerase chain reaction. American Journal of Human Genetics, Houston, v. 44, p. 388-396, 1989.

WRIGHT S. Evolution and Genetics of Populations. Chicago: University of Chicago, 1978. 511 p.

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Published

2017-12-08

Issue

Vol. 30 No. 4 (2017)

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Artigos

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