Genetic stability in interspecific hybridizations of wheat populations determined by meiotic index and pollen viability

Patrícia Frizon; Sandra Patussi  Brammer; Carolina Cardoso  Deuner; Amanda  Chechi; Maria Imaculada Pontes Moreira Lima; Pedro Luiz   Scheeren

doi:10.5007/2175-7925.2021.e79666

Authors

Patrícia Frizon Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo – UPF
Dra Sandra Patussi Brammer Empresa Brasileira de Pesquisa Agropecuária – Embrapa Trigo, Passo Fundo – RS, Brasil
Dra Carolina Cardoso Deuner Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo – UPF
Amanda Chechi Faculdade de Agronomia e Medicina Veterinária, Universidade de Passo Fundo – UPF
Maria Imaculada Pontes Moreira Lima Empresa Brasileira de Pesquisa Agropecuária – Embrapa Trigo, Passo Fundo – RS, Brasil
Pedro Luiz Scheeren Empresa Brasileira de Pesquisa Agropecuária – Embrapa Trigo, Passo Fundo – RS, Brasil

DOI:

https://doi.org/10.5007/2175-7925.2021.e79666

Abstract

The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained by the crosses between wheat commercial cultivars and synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, and they were fixed in Carnoy’s solution. The cytological slides were prepared by crushing the anthers and staining with 1% acetocarmine, and the normal tetrads and the ones with micronuclei were observed under a light microscope. For pollen viability, the ears were collected at the stage of mature pollen grain. The methodological procedure was similar to that of the tetrads, evaluating the pollen grains as viable, with little starch, with two pores, different sizes and non-viable. The pollen grain size was measured by the Axion Vision software. All the segregating populations with MI above 90% and high pollen viability (above 85%) reflected a stable meiotic behavior. About the variation in size, the CIGM90.909/BRS 179 cross showed pollen grains with 58.43 ?m and CIGM93.298/BRS Guamirim, 47.15 ?m. According to the results, wheat segregating populations were considered meiotically stable and suitable for use in wheat breeding programs and for incorporation of new important genes.

References

ARABBEIGI, M.; ARZANI, A.; SAEIDI, G. Meiotic behavior of wild, synthetic and cultivated wheats. Cytologia, Tokyo, v. 75, n. 2, p. 169-175, 2010.

ARORA, S.; STEUERNAGEL, B.; CHANDRAMOHAN, S.; LONG, Y.; MATNY, O.; JOHNSON, R.; CHEEMA, J. Resistance gene discovery and cloning by sequence capture and association genetics. Nature Biotechnology, New York, v. 10, p. 248-146, 2018.

BRAMBATTI, A.; BRAMMER, S. P.; WIETHÖLTER, P; NASCIMENTO JUNIOR, A. D. Genetic stability in triticale estimated by pollen viability. Arquivos do Instituto Biológico, São Paulo, v. 83, p. 1-7, 2016.

CARDOSO, B. M. Cytogenetic analyzes in synthetic strains of Triticum aestivum (T. durum x T. tauschii) and their crosses with wheat cultivars, aiming at the introgression of resistance to leaf rust – Rio Grande do Sul – Brasil. 2007. 99 f. Tese (Doutorado em Genética) - Universidade Federal do Rio Grande do Sul, Porto Alegre. 2007.

CORRÊA, M. G. S.; VIÉGAS, J.; SILVA, J. B.; ÁVILA, P. F. V.; BUSATO, G. R.; LEMES, J. S. Meiosis and pollen viability in the Araceae family. Acta Botanica Brasilica, Belo Horizonte, v. 19, n. 2, p. 295-303, 2005.

DA SILVA, D. F.; PIO, R.; NOGUEIRA, P. V.; OLIVEIRA SILVA, P. A. de; FIGUEIREDO, A. L. Pollen viability and quantification of pollen grains in species of fisális. Revista Ciência Agronômica, Fortaleza, v. 48, n. 2, p. 365-373, 2017.

FELDMAN, M.; LEVY, A. A. Genome evolution due to allopolyploidization in wheat. Genetics, Rockville, v. 192, n. 3, p. 763-774, 2012.

FRIZON, P.; BRAMMER, S. P.; LIMA, M. I. P. M.; CASTRO, R. L. D.; DEUNER, C. C. Genetic stability in synthetic wheat accessions: cytogenetic evaluation as a support in breeding programs. Ciência Rural, Santa Maria, v. 47, n. 4, p. 1-7, 2017.

GHORBANI, F.; ARZANI, A.; POURSIAHBIDI, M. M. Meiotic instability in Aegilops cylindrica: a comparison with stable meiosis in Triticum monococcum subsp. aegilopoides. Caryologia, Firenze, v. 68, n. 2, p. 101-108, 2015.

JAFARZADEH, J.; BONNETT, D.; JANNINK, J. L.; AKDEMIR, D.; DREISIGACKER, S.; SORRELLS, M. E. Breeding value of primary synthetic wheat genotypes for grain yield, PLoS One, San Francisco, v. 11, n. 9, p. 62-86, 2016.

LAZAR, M. D.; WORRALL, W. D.; PETERSON, G. L.; PORTER, K. B.; ROONEY, L. W.; THULEEN, N. A. Registration of ‘TAM 110’ wheat. Crop Science, Madison, v. 44, n. 1, p. 355-357, 2004.

LOVE, R. M. La citología como ayuda práctica al mejoramiento de los cereales. Revista Argentina Agronômica, Buenos Aires, v. 16, p. 1-13, 1949.

MIRZAGHADERI, G.; MASON, A. S. Broadening the bread wheat D genome. Theoretical and Applied Genetics, Berlin, v. 132, n. 5, p. 1295-1307, 2019.

OGBONNAYA, F. C.; ABDALLA, O.; MUJEEB-KAZI, A.; KAZI, A. G.; XU, S. S.; GOSMAN, N. Synthetic hexaploids: harnessing species of the primary gene pool for wheat improvement. Plant Breeding, Westport, v. 37, p. 35-122, 2013.

OLIVEIRA, L. B. P. de; PIERRE, P. M. O. Meiotic index and palynology of cherry-grove (Eugenia involucrata DC-Myrtaceae). Revista de Ciências Agroveterinárias, Lages v. 17, n. 4, p. 481-490, 2018.

PACINI, E.; GUARNIERI, M.; NEPI, M. Pollen carbohydrates and water content during development, presentation, and dispersal: a short review. Protoplasma, Heidelberg, v. 228, n. 1-3, p. 73, 2006.

POÇAS, M. E. P. Palinologia. 2004. Avaliable at <http://repositorium.sdum.uminho.pt/bitstream/1822/577/16/Cap%20I V.pdf>.

POZZOBON, M. T.; DE BEM BIANCHETTI, L.; SANTOS, S. dos; CARVALHO, S. I. C. de; REIFSCHNEIDER, F. J. B.; COSTA RIBEIRO, C. S. da. Meiotic behavior in accessions of Capsicum chinense Jacq. of the Embrapa Germplasm Bank, Brazil. Revista Brasileira de Biociências, Porto Alegre, v. 13, n. 2, p. 96-100, 2015.

POZZOBON, M. T.; SOUZA, K. R. R.; CARVALHO, S. I. C.; REIFSCHNEIDER, F. J. B. Meiosis and pollen viability in advanced pepper strains. Horticultura Brasileira, Brasília, v. 29, n. 2, p. 212-216, 2011.

ROSA, P. S.; CORRÊA, M. G. S.; NASCIMENTO, A. J.; BRAMMER, S. P. A.; VIÉGAS, J. Análise de grãos de pólen e tétrade em triticale hexaplóide. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, XV, 2006, Pelotas. Resumos... Pelotas: UFPel, 2006. Disponível em <http://www2.ufpel.edu.br/cic/2006/resumo_expandido/CB/CB_00895.pdf>.

RUDD, J. C.; DEVKOTA, R. N.; BAKER, J. A.; PETERSON, G. L.; LAZAR, M. D.; BEAN, B.’TAM 112’ wheat, resistant to greenbug and wheat curl mite and adapted to the dryland production system in the southern high plains. Journal of Plant Registrations, Madison, v. 8, n. 3, p. 291-297, 2014.

SANTOS, T. A. dos.; TIAGO, P. V.; SCHMITT, K. F. M.; MARTINS, K. C.; ROSSI, A. A. B. Pollen viability in Bertholletia excelsa Bonpl. (Lecythidaceae) based on different colorimetric tests. Enciclopédia Biosfera, Goiânia, v. 11, n. 22, p. 3136-3144, 2015.

SOARES, T. L.; SOUZA, E. H. D.; ROSSI, M. L.; SOUZA, F. V. D. Morphology and viability of pollen grains of wild pineapple accesses. Ciência Rural, Santa Maria, v. 41, n. 10, p. 1744-1749, 2011.

SOUZA, M. M.; PEREIRA, T. N. S.; MARTINS, E. R. Microsporogenesis and microgametogenesis associated with floral bud size and anther, and pollen viability in yellow passion fruit (Passiflora edulis f. flavicarpa Degener). Ciência e Agrotecnologia, Lavras, v. 26, n. 6, p. 1209 -1217, 2002.

SOUZA, M. M.; PEREIRA, T. N. S.; VIEIRA, M. L. C. Cytogenetic studies in

some species of Passiflora L. (Passifloraceae): a review emphasizing Brazilian species.

Brazilian Archives of Biology and Technology, Curitiba, v. 51, n. 2, p. 247-258, 2008.

TECHIO, V. H.; DAVIDE, L. C.; PEDROZO, C. A.; PEREIRA, A. V. Viability of the pollen grains of elephant grass accessions, millet and interspecific hybrids (elephant -grass x millet). Acta Scientiarum. Biological Sciences, Maringá, v. 28, n. 1, p. 7-12, 2006.

TONIAZZO, C.; BRAMMER, S. P.; CARGNIN, A.; WIETHÖLTER, P. Ocorrência de micronúcleos e inferência da instabilidade genética em acessos de trigos sintéticos. Online Research and Development Bulletin, 2018. 18 p. Available at <https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177641/1/ID44329-2017BPDO88.pdf>.

URIO, E. A. Caracterização citogenética clássica e molecular de trigos brasileiros – Rio Grande do Sul – Brasil. 2013. 123 f. Dissertação (Mestrado em Agronomia) - Universidade de Passo Fundo, Passo Fundo. 2013.

YANG, W.; LIU, D.; LI, J.; ZHANG, L.; WEI, H.; HU, X. Synthetic hexaploid wheat and its utilization for wheat genetic improvement in China. Journal of Genetics and Genomics, Seoul, v. 36, n. 9, p. 539-546, 2009.

ZANOTTO, M.; BRAMMER, S. P.; NASCIMENTO JUNIOR, A. D.; SCAGLIUSI, S. Pollen viability as assisted selection in the triticale breeding program. Ciência e Agrotecnologia, Lavras, v. 33, p. 2078-2082, 2009.