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Abstract
The susceptibility of two renal cell lines, one bovine cell line (GBK) and one leporine cell line (RK13) to bovine herpesvirus 1 (BHV-1, infectious bovine rhinotracheitis virus - IBR) as well as the ability of this virus to survive at low temperatures, were studied in the present paper. The infectivity of BHV - 1 was maintained during the 3 months testing period when stored at -80ºC and -20ºC. Nevertheless, samples kept at -20ºC presented lower titles than those stored at -80ºC. Samples kept at -20ºC in the presence of a cryoprotectant and samples kept at -80ºC presented similar titles. Samples from GBK and RK13 cells, stored at 4ºC, remained viable after 44 and 32 days, respectively. Furthermore, GBK were more susceptible than RK13 cells to BHV-1, independently of the storage temperature.Downloads
Published
1996-01-01
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Copyright (c) 1996 Veridiana Munford, Isabela Cristina Simoni
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